Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction.
| dc.contributor.author | Hong, H. A. | |
| dc.contributor.author | Loubser, A. S. | |
| dc.contributor.author | de Assis Rosa, Debra. | |
| dc.contributor.author | Naranbhai, Vivek. | |
| dc.contributor.author | Carr, William Henry. | |
| dc.contributor.author | Paximadis, Maria. | |
| dc.contributor.author | Lewis, David A. | |
| dc.contributor.author | Tiemessen, Caroline Tanya. | |
| dc.contributor.author | Gray, Clive M. | |
| dc.date.accessioned | 2012-11-24T07:31:31Z | |
| dc.date.available | 2012-11-24T07:31:31Z | |
| dc.date.created | 2011 | |
| dc.date.issued | 2011 | |
| dc.description.abstract | The effector function of natural killer (NK) cells is modulated by surface expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that interact with human leukocyte antigen (HLA) class I ligands. We describe the use of real-time polymerase chain reaction (PCR) assays that allow easy and quick detection of 16 KIR genes and the presence/absence of KIR-ligands based on allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. These methods overcome the tedious and expensive nature of conventional KIR genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing (SBT). Using these two cost-effective assays, we measured the frequencies of KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited in South Africa. | en |
| dc.identifier.citation | Hong H. A. et al. 2011. Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction. Tissue Antigens 78 (3), pp. 185–194. | en |
| dc.identifier.issn | 0001-2815 | |
| dc.identifier.uri | http://dx.doi.org/10.1111/j.1399-0039.2011.01749. | en |
| dc.identifier.uri | http://hdl.handle.net/10413/7998 | |
| dc.language.iso | en | en |
| dc.publisher | John Wiley & Sons. | en |
| dc.subject.other | Genotyping. | en |
| dc.subject.other | Human leukocyte antigen killer-cell immunoglobulin-like receptor-ligand. | en |
| dc.subject.other | Killer-cell immunoglobulin-like receptor. | en |
| dc.subject.other | Real-time polymerase chain reaction. | en |
| dc.title | Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction. | en |
| dc.type | Peer reviewed journal article | en |