Synthesis of DNA - protein conjugates and a preliminary study of their interaction with eukaryotic cell receptors.
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Date
1986
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Abstract
Thymidine oligomers were chemically synthesised and linked to available amino functions of transferrin in alternative orientations: (a) A CMP residue attached to the 3' end of (pT)₁₀ with terminal deoxynucleotidyl transferase was oxidised with NaI0 and linked to transferrin via a Schiff base
formation. (b) The 5' terminal phosphate group of (pT)₅ was activated with imidazole and reacted with transferrin to form a phosphoramide bond. The (pT)₅ thus attached to the protein was elongated to (pT)₃₀₀ using terminal
deoxnucleotidyl transferase and TTP. The latter conjugate was capable of hybridising poly(A) tailed linear PBR322 DNA. The binding of this hybridisation complex to the transferrin receptor on various cell types was investigated.
Description
Thesis (M.Sc.)-University of Durban-Westville, 1986.
Keywords
Biosynthesis., Thymidine., Transferrin., Theses--Biochemistry., Eukaryotic cells., Recombinant DNA., Deoxyribonucleic acid.