Browsing by Author "Nemakanga, Rendani."

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Pepper and tomato seed performance in response to imbibition and dehydration.
(2005) Nemakanga, Rendani.; Modi, Albert Thembinkosi.
The International Seed Testing Association and the Association of Official Seed analysts define seed vigour as the ability of a seed lot to perform during the germination process and crop stand establishment under a wide range of environmental conditions. There are many ways to determine seed vigour, but few satisfy the requirements of being simple, inexpensive and reproducible, among others, to permit the seed industry to adopt seed vigour as an indicator of seed quality when they sell seeds. Hence, the standard germination test, which is performed under uniform and favourable conditions, is generally used to indicate seed quality when seeds are marketed. The objective of this study was to determine the performance of tomato and pepper seeds in response to pre-germination hydration and dehydration relative humidities (12%, 49% and 75% RH). Before hydration, seeds were hydrated at three temperatures (10°C, 20°C and 30°C). Hydration was performed by imbibing pre-weighed pepper ('Santarini' and 'California Wonder'), Chilli and tomato ('Heinz', 'Marondera' and 'Roma') seeds for 2 h in 10 ml of distilled H(2)0 per 100 seeds at 10°C, 20°C and 30°C. Dehydration was performed by change in seed mass determined during a 72-hour dehydration at 12%, 49% and 75% RH. Seed performance in response to imbibition and dehydration was determined by leakage of electrolytes from seeds during imbibition, laboratory germination capacity and seedling emergence under simulated shadehouse nursery conditions. A pot experiment was conducted to determine the effects of seed treatments on yield. Seed mass increased by about 50% during the 2-hour of hydration. Dehydration was hastened by decreasing the RH, and 12% RH significantly (P < 0.05) reduced the post-imbibition seed moisture content compared with 49% and 75% RH. The latter two relative humidities reduced the seed moisture content to about 10% and 15%, respectively, for all cultivars, irrespective of imbibition temperature. Low imbibition temperature (10°C) significantly (P < 0.01) increased electrolyte leakage, compared with high imbibition temperatures (20°C and 30°C), which were not significantly different from each other. At all hydration temperatures, low RH (12%) caused a significant (P < 0.01) decrease in seed germination whereas 49% RH and 75% RH apparently had a priming effect on seeds. There was no significant difference between imbibition temperatures, with respect to seed germination, but 100G caused a significant decrease in germination index, a measure of seed vigour. Seedling emergence was significantly (P < 0.01) reduced by both low imbibition temperature (10oG) and low dehydration relative humidity (12% RH). The negative effects of low imbibition temperature and rapid dehydration at 12% RH were also observed as stunted seedling growth. Principal component analysis and linear regression were used to determine a statistical model to predict seedling emergence from germination percentage. The model predicted emergence consistently, but it overestimated it by about 2% to 3%. It is concluded that low imbibition temperature and rapid dehydration can be used to simulate stress to determine seed performance in pepper and tomato.