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Browsing by Author "Tiemessen, Caroline Tanya."

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    CCR5 expression, haplotype and immune activation in protection from infection in HIV‐exposed uninfected individuals in HIV serodiscordant relationships.
    (John Wiley & Sons., 2017) Jaumdally, Shameem Z.; Picton, Anabela.; Tiemessen, Caroline Tanya.; Paximadis, Maria.; Jaspan, Heather B.; Gamieldien, Hoyam.; Masson, Lindi.; Coetzee, David.; Williamson, Anna-Lise.; Little, Francesca.; Gumbi, Pamela Phumelele.; Passmore, Jo-Ann Shelley.
    Abstract available in pdf.
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    Epigenetic mechanisms, T-cell activation, and CCR5 genetics interact to regulate T-cell expression of CCR5, the major HIV-1 coreceptor.
    (United States National Academy of Sciences., 2015) Gornalusse, German G.; Mummidi, Srinivas.; Gaitan, Alvaro A.; Jimenez, Fabio.; Ramsuran, Veron.; Picton, Anabela.; Rogers, Kristen.; Manoharan, Muthu Saravanan.; Avadhanam, Nymisha.; Murthy, Krishna K.; Martinez, Hernan.; Murillo, Angela Molano.; Chykarenko, Zoya A.; Hutt, Richard.; Daskalakis, Demetre.; Shostakovich-Koretskaya, Ludmila.; Abdool Karim, Salim Safurdeen.; Martin, Jeffrey N.; Deeks, Steven G.; Hecht, Frederick M.; Sinclair, Elizabeth.; Clark, Robert A.; Okulicz, Jason.; Valentine, Fred T.; Martinson, Neil.; Tiemessen, Caroline Tanya.; Ndung'u, Peter Thumbi.; Hunt, Peter W.; He, Weijing.; Ahuja, Sunil K.
    Abstract available in pdf.
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    Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction.
    (John Wiley & Sons., 2011) Hong, H. A.; Loubser, A. S.; de Assis Rosa, Debra.; Naranbhai, Vivek.; Carr, William Henry.; Paximadis, Maria.; Lewis, David A.; Tiemessen, Caroline Tanya.; Gray, Clive M.
    The effector function of natural killer (NK) cells is modulated by surface expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that interact with human leukocyte antigen (HLA) class I ligands. We describe the use of real-time polymerase chain reaction (PCR) assays that allow easy and quick detection of 16 KIR genes and the presence/absence of KIR-ligands based on allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. These methods overcome the tedious and expensive nature of conventional KIR genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing (SBT). Using these two cost-effective assays, we measured the frequencies of KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited in South Africa.