Tissue culture studies on Peperomia clusifolia Hook. and Strongylodon macrobotrys A. Gray.
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Date
1982
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Abstract
Tissue culture studies were carried out on two
ornamental plant species, Strongylodon macrobotrys A. Gray
and Peperomia clusifolia Hook. Successful in vitro
regeneration of plantlets was achieved in the latter species,
using leaf and stem explants. The basal medium of Murashige
and Skoog (1962), in combination with various levels of NAA
and K, was utilised.
Strongylodon proved refractory to both establishment
of a thriving callus culture and in vitro formation of
roots and shoots. Several media were utilised, Miller's (1963)
medium proving the most successful for the production of callus.
Different combinations of the growth regulators NAA, IBA, BA
and K were used to determine optimum levels of these substances
for callus production. Root/shoot induction studies were
carried out using the basal medium of Miller (1963) plus
various concentrations of IBA in combination with K or BA.
Alternatively,the basal medium was used without added growth
regulators. Internodes, nodal segments, leaves, pulvini,
flower parts and seeds were used in the study. No plantlets
were obtained from Strongylodon explants.
Description
Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 1982.
Keywords
Plant tissue culture., Plant cell culture., Peperomia., Strongylodon., Theses--Botany.