Induction of secondary metabolite production in endophytic fungi isolated from Albizia adianthifolia using multiple small compounds involved in fungal chromatin remodeling.
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Date
2021
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Abstract
Fungi are an inexhaustible source of unexplored bioactive secondary metabolites that have been useful to
humans as pharmaceuticals and other valuable products. The unexplored arsenal of secondary metabolites
from fungi and the growing problem of antibiotic resistance have led to the search for novel bioactive
secondary metabolites in fungi from unique niches. Endophytic fungi are an unexplored niche of fungi that
have a special mutualistic relationship with their hosts. Endophytic fungi and medicinal plants' relationship
has received attention as endophytic fungi have been shown to produce bioactive secondary metabolites
with a profile similar to secondary metabolites from their host medicinal plants. Despite the potential of
endophytic fungi as an arsenal for novel pharmacological important secondary metabolites, they remain
hugely unexplored due to the cryptic or silenced nature of some biosynthetic gene clusters coding for
secondary metabolites. The genes that code for secondary metabolites are arranged in clusters known as
biosynthetic gene clusters. Some of these biosynthetic gene clusters are sequestered away from transcription
factors; thus, they are not transcribed under standard laboratory conditions. Small-molecule modifiers have
been recognized as an important way of activating these silenced genes through epigenetic regulation of
the chromatin. Thus, the present study sought to induce secondary metabolite production in nine endophytic
fungi isolated from Albizia adianthifolia using multiple small compounds (valproic acid, quercetin, sodium
butyrate, and trimethoprim) involved in fungal chromatin remodeling. The secondary metabolites were
assayed for their antibacterial activity against Staphylococcus aureus, Escherichia coli, Mycobacterium
smegmatis, Enterococcus faecalis, and Klebsiella pneumoniae. Antibacterial activity was observed from
two untreated and quercetin and trimethoprim-treated endophytic fungi identified through nucleotide
sequencing of the internal transcribed spacer of ribosomal DNA as Alternaria sp against S. aureus, E.
faecalis, and M. smegmatis. Significant antibacterial activity was observed for quercetin and trimethoprim23
treated Alternaria alternata and Alternata brassicicola. However, the valproic acid-treated A. alternata
showed slight antibacterial activity against the S. aureus, E. faecalis, and M. smegmatis, while no
antibacterial activity was observed for valproic acid-treated A. brassicicola. No antibacterial activity was
observed with sodium butyrate-treated A. alternata and A. brassicicola. The findings of this study add to
the pool of studies aimed at discovering new drugs from endophytic fungi to address antimicrobial
resistance. Future studies on high performance liquid chromatography to identify the active secondary
metabolites from A. brassicicola and A. alternata will be done. Also, future studies to fully assess the
mechanisms of chromatin remodeling in the active endophytic fungi will assist in identifying small
molecule modifiers that can activate secondary metabolite production in endophytic fungi.
Description
Masters Degree. University of KwaZulu-Natal, Durban.