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Tissue culture studies on Peperomia clusifolia Hook. and Strongylodon macrobotrys A. Gray.

dc.contributor.advisorBrown, Neville Arthur Claud.
dc.contributor.authorPeters, Deborah.
dc.date.accessioned2014-09-05T13:04:58Z
dc.date.available2014-09-05T13:04:58Z
dc.date.created1982
dc.date.issued1982
dc.descriptionThesis (M.Sc.)-University of Natal, Pietermaritzburg, 1982.en
dc.description.abstractTissue culture studies were carried out on two ornamental plant species, Strongylodon macrobotrys A. Gray and Peperomia clusifolia Hook. Successful in vitro regeneration of plantlets was achieved in the latter species, using leaf and stem explants. The basal medium of Murashige and Skoog (1962), in combination with various levels of NAA and K, was utilised. Strongylodon proved refractory to both establishment of a thriving callus culture and in vitro formation of roots and shoots. Several media were utilised, Miller's (1963) medium proving the most successful for the production of callus. Different combinations of the growth regulators NAA, IBA, BA and K were used to determine optimum levels of these substances for callus production. Root/shoot induction studies were carried out using the basal medium of Miller (1963) plus various concentrations of IBA in combination with K or BA. Alternatively,the basal medium was used without added growth regulators. Internodes, nodal segments, leaves, pulvini, flower parts and seeds were used in the study. No plantlets were obtained from Strongylodon explants.en
dc.identifier.urihttp://hdl.handle.net/10413/11149
dc.language.isoen_ZAen
dc.subjectPlant tissue culture.en
dc.subjectPlant cell culture.en
dc.subjectPeperomia.en
dc.subjectStrongylodon.en
dc.subjectTheses--Botany.en
dc.titleTissue culture studies on Peperomia clusifolia Hook. and Strongylodon macrobotrys A. Gray.en
dc.typeThesisen

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