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Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction.

dc.contributor.authorHong, H. A.
dc.contributor.authorLoubser, A. S.
dc.contributor.authorde Assis Rosa, Debra.
dc.contributor.authorNaranbhai, Vivek.
dc.contributor.authorCarr, William Henry.
dc.contributor.authorPaximadis, Maria.
dc.contributor.authorLewis, David A.
dc.contributor.authorTiemessen, Caroline Tanya.
dc.contributor.authorGray, Clive M.
dc.date.accessioned2012-11-24T07:31:31Z
dc.date.available2012-11-24T07:31:31Z
dc.date.created2011
dc.date.issued2011
dc.description.abstractThe effector function of natural killer (NK) cells is modulated by surface expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that interact with human leukocyte antigen (HLA) class I ligands. We describe the use of real-time polymerase chain reaction (PCR) assays that allow easy and quick detection of 16 KIR genes and the presence/absence of KIR-ligands based on allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. These methods overcome the tedious and expensive nature of conventional KIR genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing (SBT). Using these two cost-effective assays, we measured the frequencies of KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited in South Africa.en
dc.identifier.citationHong H. A. et al. 2011. Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction. Tissue Antigens 78 (3), pp. 185–194.en
dc.identifier.issn0001-2815
dc.identifier.urihttp://dx.doi.org/10.1111/j.1399-0039.2011.01749.en
dc.identifier.urihttp://hdl.handle.net/10413/7998
dc.language.isoenen
dc.publisherJohn Wiley & Sons.en
dc.subject.otherGenotyping.en
dc.subject.otherHuman leukocyte antigen killer-cell immunoglobulin-like receptor-ligand.en
dc.subject.otherKiller-cell immunoglobulin-like receptor.en
dc.subject.otherReal-time polymerase chain reaction.en
dc.titleKiller-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction.en
dc.typePeer reviewed journal articleen

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