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Isolation of entomopathogenic gram positive spore forming bacteria effective against coleoptera.

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dc.contributor.authorDu Rand, Nicolette.
dc.date.accessioned2010-09-20T10:58:23Z
dc.date.available2010-09-20T10:58:23Z
dc.date.created2009
dc.date.issued2009
dc.descriptionThesis (Ph.D.) - University of KwaZulu-Natal, Pietermaritzburg, 2009.en_US
dc.description.abstractFourteen spore-forming bacterial strains were isolated and screened for entomopathogenic activity. Five displayed toxicity towards the common mealworm, Tenebrio molitor L., (Coleoptera: Tenebrionidae). The majority of the isolates were obtained from insect larvae and insect rich environments. The three bacterial species identified were Bacillus thuringiensis Berliner, Brevibacillus laterosporus Laubach and Bacillus cereus Frankland and Frankland. Bioassays were conducted using T. molitor larvae. The one isolate of B. cereus required the highest concentration of bacterial cells to achieve its LC50, whereas one of the isolates of B. laterosporus required the lowest cell concentration to achieve its LC50. Dose response curves were generated for the five best isolates, which showed that the isolate of B. laterosporus (NDR2) was substantially more toxic than the other isolates.
dc.identifier.urihttp://hdl.handle.net/10413/1235
dc.language.isoen_ZAen_US
dc.subjectBacillus (Bacteria)en_US
dc.subjectBacillus thuringiensis.en_US
dc.subjectBacillus cereus.en_US
dc.subjectMicrobial insecticides.en_US
dc.subjectInsect pests--Biological control.en_US
dc.subjectBiological pest control agents.en_US
dc.subjectSugarcane--Diseases and pests--South Africa.en_US
dc.subjectTenebrionidae--South Africa.en_US
dc.subjectScarabaeidae--South Africa.en_US
dc.subjectTheses--Plant pathology.en_US
dc.titleIsolation of entomopathogenic gram positive spore forming bacteria effective against coleoptera.en_US
dc.typeThesisen_US

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Doctoral Degrees (Plant Pathology)